Adult stem cell line into which hepatocyte growth factor gene and neurogenic transcription factor gene having basic helix loop helix motif are introduced, and use thereof
The present invention relates to an adult stem cell line introduced with an HGF gene and a neurogenic transcription factor gene of a bHLH family, a preparation method of the adult stem cell line, a composition for the prevention or treatment of neurological diseases comprising the adult stem cell line, and a method for treating neurological diseases comprising the step of administering the composition to a subject having neurological diseases or suspected of having neurological diseases. The adult stem cells according to the present invention, which are introduced with an HGF gene and a neurogenic transcription factor gene of a bHLH family, can be used to overcome chronic impairment caused by cell death following stroke. Thus, the adult stem cells can be developed as a novel therapeutic agent or widely used in clinical trial and research for cell replacement therapy and gene therapy that are applicable to neurological diseases including Parkinson's disease, Alzheimer disease, and spinal cord injury as well as stroke.本発明は、ＨＧＦ遺伝子とｂＨＬＨファミリーの神経原性転写因子の遺伝子とを導入した成体幹細胞株、該成体幹細胞株の作製方法、該成体幹細胞株を含む神経疾患の予防又は治療用の組成物、及び神経疾患を患うか又は神経疾患を患う疑いのある被験体に該組成物を投与する工程を含む神経疾患を治療する方法に関する。ＨＧＦ遺伝子とｂＨＬＨファミリーの神経原性転写因子の遺伝子とを導入した本発明による成体幹細胞を使用して、脳卒中後の細胞死に起因する慢性機能障害を克服することができる。このようにして、成体幹細胞を新規の治療剤として開発するか、又はパーキンソン病、アルツハイマー病及び脊髄損傷並びに脳卒中を含む神経疾患に適用可能な細胞置換療法及び遺伝子療法の臨床試験及び研究に広く使用することができる。【選択図】図９
