Durack, Gary,Hatcher, Jeremy,Westfall, Lon A.,Helbing, David R.,Wallace, Jeffrey D.,Vandre, Gary P.,Didion, Bradley,Nayak, Niraj V.,Anzar, Muhammad,Ludwig, Cindy L.,Graham, Jeffrey A.,Crowley, Kathlee
申请号：
ES10184426
公开号：
ES2569617T3
申请日:
2004.03.29
申请国别(地区):
ES
年份:
2016
代理人:
摘要：
A method for evaluating a set of conditions for staining a cell population sorted, including population a first type and a second cell type, wherein the method includes: (a) staining a fraction of the population of cells with a fluorescent dye under one set of staining conditions (B) exposing the stained cells to electromagnetic radiation as the cells pass through an interrogation location of a flow cytometer at a rate, R (C) determining a characteristic fluorescence emission exposed cells (D) using the characteristic fluorescence emission determined to classify cells exposed to two or more subpopulations of cells, one of the subpopulations a subpopulation enriched for the first type of cells (E) determining a coefficient of variation for the characteristic fluorescence emission of the cells of the subpopulation enriched, wherein a microprocessor provides an automated estimation of the coefficient of variation to provide an indication of the efficiency of the qualifying conditions staining, where the microprocessor provides automated coefficient estimation variance by estimating statistics 2nd order distributions of subpopulations using a model of known form and finding the best fit of that model to the observed data and using algorithm parametric or non-parametric computer, and wherein the model comprises a four-component model or three-component model to estimate the characteristics of sub-populations, where sub-populations comprise populations of sperm cells X lined or populations of sperm cells And aligned and (f) determining whether to modify any of staining conditions under which have staining the cells or the rate, R, which stained cells have to pass through the location interrogation flow cytometer in order to improve the efficiency of classification (G) applying the modified staining condition to the rest of the cell population.Un procedimiento para evaluar un conjunto de condiciones para teñir una población de células a clasificar, incluyendo la población
