The present invention discloses a harmless treatment device for medical waste, comprising a box body having hollow interior, upper end provided with a disposal port; lower end provided with a first door body capable of opening and closing; a sterilization chamber provided with a first division plate inside along the disposal port, first division plate dividing sterilization chamber into a first and second chamber, a spray sterilization device and ultraviolet sterilizer disposed in first chamber, exhaust gas purification device installed in second chamber; waste storage chamber, second division plate disposed between waste storage chamber and sterilization chamber, second division plate provided with opening, upper end and lower end of opening is respectively mounted with second door body and packaging bag, conveying device of packaging bag and bag sealing device fixed under conveying device. The device can perform harmless sterilization on medical waste and realize automatic sealing and packaging.