BRADY, JAMES FRANCIS,SIMMONS, DANA PHILIP,WILSON, TIMOTHY EDWARD
申请号：
CA2393084
公开号：
CA2393084C
申请日:
2000.12.06
申请国别(地区):
CA
年份:
2013
代理人:
摘要：
The present invention provides immunogens for generating anti-neonicotinoidantibodies as well as antibodies, methods, reagents, and kits for determiningthe presence of one or more neonicotinoid insecticides in a sample byimmunoassay. The present invention has particular application to determinationof the concentration of neonicotinoid insecticides applied to a plantpropagation materials such as seed, said antibody is selective for a compoundof formula (I) wherein A is 2-chloropyrid-5-yl or 2-chlorothiazol-5-yl； R andR3 independently are hydrogen or C1-C4alkyl； R1 and R2 independently arehydrogen or C1-C4alkyl or R1 and R2 are taken together with the nitrogen atomsto which they are attached to form an imidazoline or an oxadiazine ring； and Xis N-NO2 or N-CN. Also claimed is a compound of formula (IA) and proteinconjugate of formula (II) in which PR is a protein residue of a carriermolecule selected from the group consisting of a purified protein derivative(PPD, Tuberculin) from Diptheria virus, bovine serum albumin, cationizedbovine serum albumin, human serum albumin, ovalbumin and keyhole limpethemocyanin； or an enzyme residue selected from the group consisting ofalkaline phosphatase, horseradish peroxidase, and .beta.-galactosidase.
