The invention relates to a needleless injection device, containing at least one membrane, or a corresponding device for needleless injection of a substance, having at least one membrane, with which a powdered, gel-like or liquid agent, in particular an active agent, can be injected in a needleless manner into a tissue or a body by means of high impact speed. The invention also relates to a method for such a needleless injection device containing a membrane, and use thereof.