GILLIGAN THOMAS BOYD,LENZ RICHARD,GONZALEZ-MARIN CLARA,VISHWANATH RAMAKRISHNAN,EVANS KENNETH MICHAEL
申请号：
NZ63038013
公开号：
NZ630380A
申请日:
2013.03.04
申请国别(地区):
NZ
年份:
2016
代理人:
摘要：
A method of processing sperm comprises a) adjusting the concentration of a sperm sample to a predetermined concentration range and adjusting the pH of the sperm sample towards a predetermined pH by extending the sperm sample in an initial extender, centrifuging the extended sperm sample and removing supernatant until the predetermined concentration is reached and b) staining the reconcentrated sperm sample with a single staining buffer having a DNA selective dye and a quenching dye. A method of sorting sperm comprises extending a sperm sample in an initial extender having a first pH； centrifuging the extended sperm sample and removing supernatant to reach a predetermined concentration range； staining the sperm sample with a staining buffer having a DNA selective dye at an elevated pH； staining the concentrated sperm sample with a quenching dye and sorting the stained sperm sample, where the elevated pH introduced with the DNA selective dye is maintained until the time of sorting.
