The polynucleotide the magnesium in producing the constituent for remedy which the transfect it can point in the cell (Ma2+) useful constituent null magnesium (the Mg 2+ )
PROBLEM TO BE SOLVED: To dramatically improve the transfection efficiency by adding magnesium to a therapeutic composition for transfecting a polynucleotide into a vertebrate tissue.SOLUTION: Magnesium(Mg2+) is added to a therapeutic composition for in vivo transfection of a polynucleotide into a cell. The magnesium is preferably magnesium chloride. The therapeutic composition preferably contains 0.1-100 mM, preferably 0.1-10 mM of the magnesium. The therapeutic composition is preferably used for administration to a target cell (preferably muscle) of a vertebrate. The composition preferably contains the polynucleotide and the concentration of the polynucleotide in the composition is preferably 0.1 &mug/ml to 20 mg/ml. Thereby, the composition useful for gene therapy, vaccination, etc., can be obtained.COPYRIGHT: (C)2000,JPO(修正有)【解決手段】 in vivoにおいて、細胞へポリヌクレオチドを導入するための、治療用組成物の製造における、マグネシウム(Mg2+)の使用。【効果】 上記の組成物は遺伝子治療、ワクチン接種、および遺伝子ペースの生成物が、in vivoで細胞に投与される治療または予防状況のいずれにも有用である。
