The present invention relates to a method for production of Japanese encephalitis vaccine. The method includes inoculating cells for vaccine production on a cell culture carrier, culturing the cells in a tidal culture system for 3 to 10 days, inoculating the Japanese encephalitis virus (JEV) into the cells, culturing the inoculated cells for 3 to 10 days, harvesting the virus, and purifying and inactivating the virus to obtain inactivated JEV vaccine. The present invention also relates to an inactivated JEV vaccine prepared by the method above.本發明係關於一種日本腦炎疫苗之製備方法，包括將製造疫苗用的細胞接種於一細胞培養載體上，於一潮汐式培養系統中培養3~10天後，再將日本腦炎病毒接種至該細胞上，並培養3~10天後多次收穫病毒液，進行純化及不活化處理，以得到日本腦炎去活化病毒疫苗。本發明係關於一種如上述之方法所製備之日本腦炎不活化病毒疫苗。
