The present disclosure relates to a bioprinter printing assembly and a bioprinter, wherein the printing assembly comprises: a support body and a rotary rod provided with a nutrient solution supply port (14), wherein the rotary rod is rotatably supported on the support body, a surface of the rotary rod is used for receiving bio-ink provided by a printing spray head, the rotary rod includes a hollow tube section (22) having a central through hole, and a tube wall of the hollow tube section (22) is provided with a permeable hole (26) communicating with the central through hole, and the nutrient solution supply port communicates with the central through hole of the rotary rod to guide external nutrient solution into the rotary rod. The bioprinter printing assembly of the present disclosure can guide the external nutrient solution into the inside of the rotary rod through the nutrient solution supply port in the printing process, and then exudes from the permeable hole to supply the cells and biological tubular tissues on the rotary rod, thereby improving the survival rate and biological function of the cells.
