THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY
发明人:
COCHRAN, JENNIFER R.,JONES, DOUGLAS S.,KARIOLIS, MIHALIS S.,TSAI, PING-CHUAN
申请号：
CA2817197
公开号：
CA2817197A1
申请日:
2011.11.07
申请国别(地区):
CA
年份:
2012
代理人:
摘要：
The present disclosure presents a general approach to engineering existing protein-protein interactions through domain addition and evolution. The disclosure teaches the creation of novel fusion proteins that include knottin peptides where a portion of the knottin peptide is replaced with a sequence that has been created for binding to a particular target. Such fusion proteins can also be bispecific or multi specific in that they can bind to and/or inhibit two or more receptors or receptor ligands. Knottins may be fused with an existing ligand (or receptor) as a general platform for increasing the affinity of a ligand-receptor interaction or for creating a multi specific protein. In addition, the fusion proteins may comprise a knottin peptide fused to another protein where the other protein facilitates proper expression and folding of the knottin.
