The present invention relates, in some embodiments thereof, to devices and methods for decontaminating a surface of one or more vessels. In some embodiments, the devices of the invention include a first and a second chamber wherein the first chamber configured to accommodate a sterilizing substance and is fluidly connected to the second chamber, the second chamber configured to connect at least one vessel and to receive the sterilizing substance from the first chamber, wherein upon transferring the sterilizing substance to the second chamber, the surface of the at least one vessel is decontaminated.
