A cultivation method for mushrooms is disclosed. A light-emitting diode module, which comprises a plurality of light-emitting diode chips therein provided with different wavelengths, is mainly utilized as a light source arranged above the cultivated mushrooms. A controller is utilized to set the output wavelength combination, the output power and the output time of each light-emitting diode chip of the light-emitting diode module. Accordingly, the mushrooms can rapidly grow up under an effective specific wavelength, therefore, are capable of attaining an excellent safety mass and an increased quantity of nutrient ingredients.本發明之培育方法主要於所栽培之菇菌上方利用發光二極體模組作為光源,該發光二極體模組內具有複數不同波長之發光二極體晶片,利用一控制器可設定該發光二極體模組內各發光二極體晶片輸出之波長組合、輸出功率以及輸出時間,讓菇菌可在有效的特定波長下迅速生長,達到良好的安全質量且增加其營養成分之含量。1‧‧‧培育裝置10‧‧‧培育架12‧‧‧隔板20‧‧‧發光二極體模組40‧‧‧菇菌培育單元