University of Central Florida Research Foundation; INC.
发明人:
Daniel Henry,Debopam Chakrabarti
申请号：
BRPI0818268
公开号：
BRPI0818268A2
申请日:
2008.10.31
申请国别(地区):
BR
年份:
2017
代理人:
摘要：
Disclosed is a method of making a malaria vaccine, the method comprising stably transforming a plant by inserting into its plastid genome a nucleic acid sequence encoding and operable to constitutively express a malaria antigenic polypeptide selected from AMA-1, MSP-1 or both； harvesting the stably transformed plant in whole or in part； purifying the expressed malaria antigenic polypeptide from the harvested plant； and packaging the purified antigenic polypeptide under sterile conditions in an amount for a predetermined dosage. Also disclosed is an oral vaccine effective in raising malaria antibodies in a susceptible host, the vaccine comprising leaf material from an edible plant containing plastids stably transformed to constitutively express a fusion polypeptide consisting essentially of cholera toxin B subunit and a malaria antigenic polypeptide selected from AMA-1, MSP-1 or both.
