ДОУЛЛИНГ Вивиан В. (US),ПОСТОН Ребекка М. (US),ХЕГГЕН-ПИЙ Черилин Л. (US),АВАКЯН Алан П. (US),ТИЧКОВСКИ Юлиус (US)
申请号：
RU2010136627/15
公开号：
RU2010136627A
申请日:
2010.08.31
申请国别(地区):
RU
年份:
2012
代理人:
摘要：
1. A method of immunizing a bird against infection with Clostridium bacteria, comprising administering in ovo during the last quarter of the incubation period an effective immunizing dose of a composition that induces an immune response against bacteria of the species Clostridium, where the immunogenic composition is administered by in ovo injection directly into the embryo, and where indicated the immunogenic composition provides Clostridium perfringens toxoid, Clostridium perfringens bacterin, Clostridium perfringens toxin other than alpha toxin, and attenuated Clostridium perfringens bacteria, or any combination thereof. ! 2. The method according to claim 1, where the immunogenic composition is introduced into the amnion. ! 3. The method according to claim 2, where the immunogenic composition is introduced axially through the large end of the egg into the amniotic fluid. ! 4. The method according to claim 1, where the immunogenic composition is introduced directly into the body of the embryo. ! 5. The method according to claim 1, where the immunogenic composition is administered parenterally to the embryo. ! 6. The method according to claim 1, where the bird is a chicken. ! 7. The method according to claim 1, where the immunogenic composition is administered on the 15-20 day of incubation. ! 8. The method according to claim 1, where the immunogenic composition is administered on the 18th or 19th day of incubation. ! 9. The method according to claim 1, where the bird is a turkey. ! 10. The method according to claim 1, where the immunogenic composition comprises an emulsion of water-oil-water. ! 11. The method according to claim 1, where the immunogenic composition comprises an adjuvant. ! 12. The method of claim 11, wherein the adjuvant comprises an aluminum derivative, saponin, oil, or any combination thereof. ! 13. The method according to claim 1, further comprising introducing in ovo an immune stimulant at any time during incubation. ! 14. The method according
