The invention provides a method and its application for promoting nature sex-changing of the grouper by human induction. The method is to take advantage of the principle of environmental adjustment factor (community control) to guide and promote nature sex-changing of a female grouper. The method is to select a healthy female grouper whose gonad is already matured. Putting female grouper to breed in a water body environment at least four female groupers per 1000 m &Lgr 3. After the grouper changes the sex into a male grouper naturally, the male grouper which changes its sex moves out. The environment can be for a female grouper to conduct the sex-changing process again.本發明係提供一種人工誘導促進石斑魚類自然性轉變之方法及其應用,該方法係利用環境調節因素(社群控制)的原理去誘導並促進一雌性石斑魚自然發生性轉變,該方法係在繁殖季節前一預定時間選擇健康且生殖腺已屆成熟之雌性石斑魚,並將至少4尾該雌性石斑魚放養於1000m3水體之環境中,等到其中一該石斑魚自然發生性轉變成為一雄性石斑魚後,則可將發生性轉變之該雄性石斑魚移出,然後再加入一尾雌魚以維持放養密度,該環境可再次使一雌性石斑魚發生性轉變。101‧‧‧在石斑魚繁殖季節前,選擇生殖腺已屆成熟之一雌性石斑魚102‧‧‧將至少4尾該雌性石斑魚放養於1000m3水體之環境中103‧‧‧將發生性轉變之該雄性石斑魚移出該環境