An anti-microbial composition is provided. The anti-microbial composition comprises an active component A and an active component B. The active component A is a compound represented by formula (I), and the active component B is a mancozeb. The weight ratio of the two components is 1:1-400. A method for preparing the composition and uses thereof are also provided. Experimental results showed that the anti-microbial composition has a significantly improved performance and, more importantly, a reduced application volume, thereby lowering application costs. The anti-microbial composition can effectively prevent and treat certain fungal diseases in a crop plant. The invention compounds anti-microbial agents with different mechanisms and modes of actions to significantly lower the application volume of each of the agents. The invention also provides excellent effects to broadening an anti-microbial spectrum, inhibiting fungal growth, improving fungal resistance, enhancing crop disease prevention and treatment, and the like.
