A method of generating a chronic myelogenic leukemia (CML) acquired chemoresistant culture model is provided. Such a method may comprise providing a naïve blast crisis CML cell line administering/contacting the cell line with a mutation-inducing dose of imatinib maintaining a culture of the treated cell line for a period of time until the treated cell line relapses and repopulates the culture and determining the repopulated cell culture is a CML acquired chemoresistant cell line by detecting a BCR-ABL mutation, wherein the acquired chemoresistance is achieved by a BCR-ABL mutation.
