Hughes, Erik Heller,Bahler, Brian Douglas,Lee, Tsu-Shun
申请号：
AU2007307800
公开号：
AU2007307800C1
申请日:
2007.10.09
申请国别(地区):
AU
年份:
2014
代理人:
摘要：
The present invention provides improved methods for the reduction or removalof protein impurities from a complex cellular Streptococcus pneumoniae lysateor centrate comprising serotype 3 polysaccharides involving steps relatingto post-lysis heating or pH adjustment. In certain methods, the lysate is heatedfor a time and at a temperature sufficient to denature proteins present in thelysate and cause their aggregation and precipitation. In one embodiment, thelysate is heated to at least 60°C for at least 30 minutes to cause proteinaggregation and precipitation, more particularly about 60°C to about70°C for about 30 to about 50 minutes, and even more particularly about 65°Cfor about 40 minutes. In other methods, the pH of the lysate or centrate is increasedto at least 8.0 to improve filterability, more particularly about 8.0 to 8.4,and even more particularly about 8.2. In further methods, heating and pH adjustmentsteps are combined to cause the aggregation and precipitation of proteins aswell as to improve filterability of the lysates or centrates. In other methods,the pH of the lysate or centrate is lowered to about 3.0 to about 5.0 to cause proteinaggregation and precipitation. Such methods allow for the production of substantiallypurified serotype 3 polysaccharide-containing lysates or centrates.
