The present disclosure relates to systems, methods and compositions for single molecule electronic sequencing of template nucleic acids using nanosensors. The nanosensors of the invention improve the measurement of polynucleotides by assimilating authentic polynucleotide binding proteins (“PBPs”) in place of conventional pore-forming proteins that do not normally interact with polynucleotides. The PBPs of the present invention form the constriction sites of electroconductive pores of the nano sensors, while maintaining their natural polynucleotide binding and processing activities.
