PURPOSE: A method for repetitively over-ovulating and a method for repetitively obtaining fertilized eggs using Bombina orientalis are provided to obtain a large amount of healthy ova and embryos more stably by minimizing stress applied to Bombina orientalis even though repetitive ovulations, thereby remarkedly increasing survival rate of adults.CONSTITUTION: A method for repetitively over-ovulating using Bombina orientalis comprises the steps of: inducing superovulation by injecting human chorionic gonadotropin (hCG) to Bombina orientalis obtaining ova from the Bombina orientalis that is induced to superovulation giving the Bombina orientalis that is induced to superovulation a rest and inducing superovulation again by injecting hCG to Bombina orientalis that had a rest. A method for repetitively production of fertilized eggs from Bombina orientalis comprises the steps of: inducing superovulation by injecting hCG to Bombina orientalis inducing spawning of ova and fertilization of the Bombina orientalis that is induced to superovulation in order to form fertilized eggs obtaining the fertilized eggs giving the Bombina orientalis that is induced to spawning a rest and inducing superovulation by injecting hCG to Bombina orientalis that had a rest again, inducing spawning of ova again and obtaining fertilized eggs again.COPYRIGHT KIPO 2013[Reference numerals] (AA) 22 - 23°C 12 times light and shade cycle (BB) Prepare entity through culture (CC) Toxicology (DD) Embryology (EE) Culture condition (FF) Entity processing (GG) Culturing method (HH) Red-bellied frog artificial ovulation induction (II) Serial No. check (JJ) Entity selection (KK) Example (LL) Optimizing (MM) Proper (NN) Hormone injection (0.1 IU/&lambda, 2 IU/&lambda) (OO) Lymphatic gland on a back side (PP) Abdominal cavity (QQ) Natural matings (RR) HCG: ? 300 IU, ? 500 IU space: braid (SS) HCG: ? 300 IU, ? 10 · 300 IU space: separation 24h later braid (TT) HCG: ? 300 IU, ? 10 · 300 IU spac
