Compositions comprising 5-cholesten-3, 25-diol, 3-sulfate (25HC3S) or pharmaceutically acceptable salt thereof and at least one cyclic oligosaccharide, e.g., a cyclodextrin (CD), are provided. The compositions may be used to prevent and/or treat a variety of diseases and conditions, including organ failure (e.g. acute liver failure), high cholesterol/high lipids, and various inflammatory diseases and conditions.提供包含5-膽甾烯-3,25-二醇3-硫酸酯(25HC3S)或其醫藥上可接受之鹽及至少一種環狀寡醣(例如環糊精(CD))的組成物。該組成物可用於預防及/或治療各種疾病及病況,包括器官衰竭(例如急性肝衰竭)、高膽固醇/高脂質和各種發炎性疾病及病況。