System and method of photoaltering a region of an eye using an enhanced contrast between the iris and the pupil of the imaged eye. The system includes a laser assembly outputting a pulsed laser beam, a user interface displaying one of a first digital image of the eye and a second digital image of the eye, and a controller coupled to the laser assembly and the user interface. The first digital image has a first contrast between the pupil and the iris, and the second digital image has a second contrast between the pupil and the iris. The controller selectively increases the first contrast between the pupil and the iris to the second contrast between the pupil and the iris and directs the pulsed laser beam to the region of the eye based on one of the first and second digital images.
