A novel multispecific antigen-binding molecule, which comprises a domain containing an antibody variable region having an activity of binding to glypican 3 and a domain containing an antibody variable region having an activity to bind to a T cell receptor complex and retains excellent cytotoxic activity and high safety, was found. The molecule according to the present invention exhibits potent cytotoxic activity on cells and tissues expressing glypican 3 and, therefore, enables the production of new pharmaceutical compositions for treating or preventing various kinds of cancers.本發明找到一種新的多專一性抗原結合分子，其包含:含有具Glypican3結合活性之抗體可變區之域、及含有具T細胞受體複合體結合活性之抗體可變區之域，且維持優良的細胞傷害活性與高安全性。本發明之分子對於表現Glypican3之細胞、組織呈強細胞傷害性，故能製造用以治療或預防各種癌之新的醫藥組合物。
