The present invention provides isolated polynucleotide sequences encoding α-mannosidase. The present invention further provides DNA constructs comprising the polynucleotide sequence coding for α-mannosidase in sense or anti-sense orientation, RNAi constructs, recombinant vectors comprising the constructs, and host cells comprising the recombinant vector. The present invention further provides transgenic plants, plant cells, transgenic progeny and seeds expressing the polynucleotide with reduced α-mannosidase protein accumulation, having enhanced fruit shelf life.