The disclosure relates to gene expression regulatory sequences from Sorghum and Setaria, specifically to the regulatory regions of GOS2 gene and fragments thereof and their use in modulating the expression of one or more heterologous nucleic acid fragments in plants. The disclosure further discloses compositions, polynucleotide constructs, transformed host cells, plants and seeds containing the recombinant construct with the promoter, and methods for preparing and using the same.
