The invention is directed to anti-frozen agent and the process for its preparation, wherein the anti-frozen agent is used for protection of biological tissues during freezing process. The process for preparing the anti-frozen agent comprises mixing ethylene glycol, water and dimethyl sulfoxide well to obtain a base, and then slowly adding sodium polyacrylate to the base. The anti-frozen agent as obtained is effective in decreasing the damage of biological tissues during the freezing treatment of the biological tissues. In particular, it can be used for preparation of recycled autografts.本發明關於一種保護生物組織在冷凍處理過程中不受破壞的防凍劑及其製備方法。本發明製備抗凍劑之方法包含將乙二醇、水及二甲基亞碸三者均勻混合製成基質;再緩慢加入聚丙烯酸鈉而得。所製得的抗凍劑在冷凍處理過程中塗覆於該生物組織,可有效減少生物組織之破壞,尤其可用於回收自體骨移植物之製備。
