PROBLEM TO BE SOLVED: To provide a technique for directly identifying the genotype at locus Rf17 on the basis of the specific base sequence data thereof, and to provide a technique for artificially constructing a fertility restoration line.SOLUTION: The invention provides a method of restoring the fertility of CW-type cytoplasmic male sterile rice by inhibiting or reducing the expression of a gene comprising a specific base sequence, and a base sequence which can recover the fertility of CW-type cytoplasmic male sterility rice which is a nucleic acid having a base sequence selected from the following groups: (a) a base sequence having at least base sequences at positions 1611th to 4835th in other specific base sequences (4871 base length) and (b) a base sequence showing 95% or more sequence identity to the base sequence (a).COPYRIGHT: (C)2015,JPO&INPIT【課題】Rf17遺伝子座の遺伝子型をその具体的な塩基配列情報に基づき直接同定する技術、及び、人為的に稔性回復系統を作成する技術を提供する。【解決手段】CW型細胞質雄性不稔性のイネにおいて、ある特定の塩基配列から成る遺伝子の発現を抑制又は減少することにより、該イネの稔性を回復させる方法、及び、以下の群から選択される塩基配列を有する核酸:(a)他の特定の塩基配列(4871塩基長)における少なくとも1611~4835番目の塩基配列を有する塩基配列;及び(b)塩基配列(a)と95%以上である配列相同性を示す塩基配列であって、CW型細胞質雄性不稔性イネの稔性を回復させることのできる塩基配列。【選択図】図1
