CONSORTIUM OF THE RECOMBINANT INFLUENZA A VIRUSES FLU-NS1-124- L7/L12-H5N1, FLU-NS1-124-OMP16-H5N1, FLU-NS1-124-L7/L12-H1N1 AND FLU-NS1- 124-OMP16-H1N1, FAMILY ORTOMYXOVIRIDAE, GENUS INFLUENZAVIRUS, EXPRESSING BRUCELLA IMMUNODOMINANT PROTEINS DESTINED TO GENERATE A VACCINE AGAINST BRUCELLOSIS
THE REPUBLICAN GOVERNMENT ENTERPRISE ON THE BASIS OF ECONOMIC CONTROL RIGHTS "RESEARCH INSTITUTE FOR BIOLOGICAL SAFETY PROBLEMS" OF THE SIENCE COMMITTEE OF THE MINISTERY OF EDUCATION AND SCIENCE OF TH
发明人:
SANSYZBAY, Abylay Rysbayuly,TABYNOV, Kaisar Kazybayevich,YESPEMBETOV, Bolat Amanbayevich,SULTANKULOVA, Kulyaisan Turlybayevna,KHAIRULLIN, Berik Mikhitovich,SANDYBAYEV, Nurlan Tamambayevich,YEGOROV, An
申请号：
KZKZ2014/000006
公开号：
WO2014/200325A2
申请日:
2014.05.23
申请国别(地区):
WO
年份:
2014
代理人:
摘要：
This invention relates to the fields of biotechnology, veterinary science and public health, and describes a vector vaccine which can be used to prevent brucellosis. The essence of the invention is that reverse genetics methods have been used to construct novel, genetically-stable recombinant influenza viral vectors based on the influenza A viral vectors Flu-NSl-124-L7/L12- H5N1, Flu-NSl-124-Omp16-H5Nl, Flu-NSl-124-L7/L12-HlNl and Flu-NSl-124-Omp16- H1N1 (of the subtypes H5N1 and H1N1) that contain the genetic sequences of the Brucella proteins L7/L12 (ribosomal) or Omp16 (outer membrane) inserted into the viral NS1 gene, which express these Brucella proteins in vivo and are safe in cattle. Our studies showed that this vaccine candidate in cattle induced a strong antigen-specific T-cell immune response, and most importantly provided a high level of protectiveness comparable to the commercial B. abortusS19 vaccine and superior to the B. abortusS19 vaccine in combination with Montanide Gel01 adjuvant. Furthermore it is shown that the developed vaccines can effectively differentiate vaccinated animals from infected animals.Cette invention concerne les domaines de la biotechnologie, de la science vétérinaire et de la santé publique, et décrit un vaccin vectoriel qui peut être utilisé pour prévenir la brucellose. Lessence de cette invention est que des méthodes de génétique inverse ont été utilisées pour construire de nouveaux vecteurs viraux grippaux recombinés, génétiquement stables basés, sur les vecteurs viraux Flu-NSl-124-L7/L12- H5N1, Flu-NSl-124-Omp16-H5Nl, Flu-NSl-124-L7/L12-HlNl et Flu-NSl-124-Omp16-H1N1 (des sous-types H5N1 et H1N1) de linfluenza A qui contiennent les séquences génétiques des protéines L7/L12 (ribosomales) ou Omp16 (membrane extérieure) de Brucella insérées dans le gène viral NS1, qui expriment ces protéines de Brucellain vivo et sont sans danger chez le bétail. Nos études ont montré que ce vaccin candidat a induit chez le bétail une forte répo
