TRUSTEES OF BOSTON UNIVERSITY;BOSTON MEDICAL CENTER CORPORATION
发明人:
MURPHY, GEORGE, J.,SHERR, DAVID, H.,ROZELLE, SARAH, S.,SMITH, BRENDEN, W.
申请号：
EP13829847
公开号：
EP2909313A4
申请日:
2013.08.15
申请国别(地区):
EP
年份:
2016
代理人:
摘要：
This disclosure provides methods of making a megakaryocyte-erythroid progenitor cell (MEP), comprising differentiating a stem cell into a MEP in culture in the presence of an aryl hydrocarbon receptor (AhR) agonist. In some embodiments the stem cell is a pluripotent stem cell. In some embodiments the MEP co-expresses CD41 and CD235. In some embodiments the number of MEPs produced in the culture increases exponentially. Methods of making a red blood cell (RBC) by culturing a MEP in the presence of an AhR agonist are also provided. Methods of making a megakaryocyte and/or a platelet, comprising culturing a MEP in the presence of an AhR modulator are also provided. In some embodiments the AhR modulator is an AhR antagonist. This disclosure also provides compositions comprising at least 1 million MEPs per ml and compositions in which at least 50% of the cells are MEPs.
