The invention relates to medicine, namely to a method for diagnosis of viral hepatitis B with the determination of AgHBs and its subsequent confirmation in persons with accidental exposure such as medical personnel, drug users, patients undergoing hemodialysis treatment, etc.Summary of the invention consists in that in two strips with wells is used AgHBs free human serum, human serum negative to AgHBs antibodies, serum with antibodies to AgHBs with a titer of more than 100 IU/ml and serum of the human being tested in the first strip, and in the second strip is used AgHBs free human serum, fetal bovine serum for calibration, inactivated human serum, containing calibrated AgHBs and serum of the human being tested. After incubation at a temperature of 18…24°C, for 30 min, diluted conjugate 1:20 of rabbit or mouse anti-species globulin and horseradish peroxidase is added in all samples, then the samples are incubated at a temperature of 37°C, for 120 min. Further, a chromogen solution containing 0.02% hydrogen peroxide solution, 4% dimethylsulfoxide solution and 0.03% tetramethylbenzidine solution is added and incubated at a temperature of 18…24°C, for 30 min, after which the optical density values are determined by the photometric method at the wavelength of 450…620 nm.Invenţia se referă la medicină, şi anume la o metodă de diagnostic al hepatitei virale B cu determinarea AgHBs şi confirmarea lui ulterioară la persoanele cu expunere accidentală, cum sunt personalul medical, utilizatorii de droguri, pacienţii aflaţi în tratament de hemodializă etc.Esenţa invenţiei constă în aceea că în două stripuri cu godeuri se utilizează ser uman ce nu conţine AgHBs, ser uman negativ la anticorpii AgHBs, ser cu anticorpi la AgHBs cu un titru mai mare de 100 UI/ml şi ser de la persoana investigată în primul strip, iar în al doilea strip se utilizează ser uman ce nu conţine AgHBs, ser fetal de bovine pentru calibrare, ser uman inactivat, care conţine AgHBs calibraţi şi ser de la persoa
