The present disclosure provides methods of improving disease tolerance of a crop plant by improving and/or inducing long-term disease tolerance of the crop plants against one or more of fungal, bacterial, and viral diseases. The methods provided in the present disclosure have desirable properties and related advantages compared to other methods known in the art.本揭露提供藉由改善和/或誘導作物植物對抗一種或多種真菌、細菌和病毒病害的長期病害耐受性來改善作物植物的病害耐受性之方法。與本領域已知的其他方法相比,本揭露中提供的方法具有希望的性質和相關優點。