Marco Alexander Van Den Berg,Marcella Katharina Madern,Remko Tsjibbe Winter
申请号：
US15516854
公开号：
US10472661B2
申请日:
2015.10.08
申请国别(地区):
US
年份:
2019
代理人:
摘要：
The present invention relates to a method for increasing the degree of glycosylation of a composition comprising steviol glycosides, which method comprises: a. contacting said composition comprising steviol glycosides with a recombinant microorganism, a cell free extract derived from such a microorganism or an enzyme preparation derived from either thereof； and b. thereby to increase the degree of glycosylation of the composition comprising steviol glycosides, wherein the recombinant microorganism comprises one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity； a polypeptide having ent-Kaurene synthase activity； a polypeptide having ent-Kaurene oxidase activity； a polypeptide having kaurenoic acid 13-hydroxylase activity； and one or more polypeptides having UDP-glucosyltransferase activity whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least one steviol glycoside. The present invention also relates to a composition comprising steviol glycosides obtainable by such a method.
