PROCESS OF OBTAINING A FUNCTIONAL DERMAL SUBSTITUTE FROM A DECELLULARIZED AMNIOTIC MEMBRANE FROM PLACENTA IN COMBINATION WITH KERATINOCYTES AND THEIR USE AS TISSULAR SKIN REGENERATION AGENT
process for obtaining a functional dermal substitute from decellularized amniotic membrane from the placenta in combination with keratinocytes and its use as a skin tissue regeneration agent the present invention provides a process for obtaining a functional dermal substitute from decellularized amniotic membrane from placenta in combination with mammalian keratinocytes and its use as a skin tissue regeneration agent, which consists of a process for the generation of an artificial skin graft in the laboratory using specialized techniques in biotechnology, such as the cultivation of mammalian cells , preferably human, in which the keratinocytes that form part of the epidermal layer of the skin are cultured under aseptic conditions, which are combined with a collagen-rich mesh, which is obtained from cells that make up the structure of the skin. skin in combination with amniotic membrane placenta to generate two layers, which can be used in burned patients or with skin lesions； additionally, this technology considerably reduces the cost of treatment compared to the cost of current methods, such as skin graft treatments (autografts or allografts) and the result of this process is prompt cellular reorganization, re- modeling and reepithelization of the wound, causing the formation of new skin tissue and avoiding the formation of fibrotic tissue resulting from muscle contraction.processo de obtenção de um substituto dérmico funcional a partir de membrana amniótica descelularizada proveniente de placenta em combinação com queratinócitos e seu uso como agente de regeneração tissular de pele a presente invenção fornece um processo de obtenção de um substituto dérmico funcional a partir de membrana amniótica descelularizada proveniente de placenta em combinação com queratinócitos de mamífero e seu uso como agente de regeneração tissular de pele, o qual consiste em um processo para a geração de um enxerto de pele artificial em laboratório utilizando técnicas especializadas em
