A complex comprising RNA and a positively charged modified polysaccharide selected from starch, amylose, amylopectin, galactan, chitosan, or dextrin, pharmaceutical composition comprising it, and its use in RNA transfection are provided. Methods for RNA transfection, gene therapy and treatment of a disease, disorder or condition comprising using the complex are also provided. The use of positively charged modified polysaccharide selected from starch, amylose, amylopectin, galactan, chitosan, or dextrin, in RNA transfection into cells is further provided.Linvention concerne un complexe comprenant de lARN et un polysaccharide modifié chargé positivement choisi parmi lamidon, lamylose, lamylopectine, le galactane, le chitosane ou la dextrine, une composition pharmaceutique le comprenant, et son utilisation dans la transfection dARN. Linvention concerne des procédés de transfection dARN, de thérapie génique et de traitement dune maladie, dun trouble ou dun état comprenant lutilisation du complexe. Linvention concerne en outre lutilisation dun polysaccharide modifié chargé positivement choisi parmi lamidon, lamylose, lamylopectine, le galactane, le chitosane ou la dextrine, dans la transfection dARN dans des cellules.
