The present invention relates to a method of collecting semen from the epididymis or the testis of a lab animal and an artificial insemination method thereof. The present invention relates to a new applicable method in the animal production field using artificial insemination. Since the present invention solves the problems of the conventional method of artificial insemination of lab animals, it is excellent in terms of the pregnancy rate and productivity is time-efficient and is superior in economic and industrial respects by preventing a huge economic loss due to the costs for maintaining the mass breeding of animals.
