The subject invention provides a method for promoting wound healing and preventing an infection or treating an existing infection with negative pressure wound therapy, said method comprising the steps of providing a sterile disinfectant composition comprising an active agent comprising chlorhexidine and administering the sterile disinfectant composition to the wound site prior to, during and/or following negative pressure wound therapy. The subject invention also provides a kit comprising the sterile disinfectant composition and, optionally, devices for administration of the sterile disinfection composition to the site with negative pressure wound therapy.