Wake Forest University;University of Pittsburgh - Of the Commonwealth System of Higher Education
发明人:
Mark T. Gladwin,Daniel B. Kim-Shapiro,Mauro Tiso
申请号：
US14795115
公开号：
US20150306183A1
申请日:
2015.07.09
申请国别(地区):
US
年份:
2015
代理人:
摘要：
Described herein is the finding that a mutant form of human neuroglobin (H64L) with a stable five-coordinate geometry reduces nitrite to nitric oxide approximately 2000-times faster than the wild type neuroglobin. Five-coordinate neuroglobin is also capable of binding and releasing oxygen. Based on these findings, the use of five-coordinate neuroglobin as a blood substitute is described herein. Particularly provided is a method of replacing blood and/or increasing oxygen delivery to tissues in a subject by administering to the subject a therapeutically effective amount of neuroglobin with a stable five-coordinate geometry. In some cases, five-coordinate neuroglobin is administered in combination with another therapeutic agent or composition, such as a second blood replacement product (for example, a hemoglobin-based oxygen carrier), a blood product (such as red blood cells, serum or plasma) or whole blood.
