CHUNGBUK NATIONAL UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION;KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY;Hong-Jun Lee
发明人:
Sang-Hoon Cha,Hong-Jun Lee,Nakwon Choi,Hong-Nam Kim
申请号：
US15011385
公开号：
US10030219B2
申请日:
2016.01.29
申请国别(地区):
US
年份:
2018
代理人:
摘要：
Provided are a neurovascular unit (NVU)-on-a-chip and a method of fabricating the same, which 3-dimensionally integrates various human brain cells in a microfluidic platform by using a brain cell co-culture technique so as to simulate a similar environment to the human brain in vitro. The NVU-on-a-chip includes an extracellular matrix (ECM) simulation material (70) in a gel state； and at least one channel (75) which passes through the ECM simulation material (70) and perfuses a culture medium, in which the ECM simulation material (70) contains a plurality of types of human brain cells on an outer side of the channel (75), a brain microvessel endothelial cell lining (91) is formed on an inner side of the channel (75), and the plurality of types of human brain cells and the brain microvessel endothelial cell lining (91) contact each other through the channel (75) to simulate a blood brain barrier (BBB) of a human brain and a neurovascular unit (NVU) of the human brain including the BBB.
