A biological image acquisition device is provided, which includes a first image detecting element, a second image detecting element, a first optical filter element, a second optical filter element and a processing unit. The first image detecting unit is used to perform optical detection on a biological tissue to obtain first detected image data. The second image detecting unit is used to perform optical detection on the biological tissue to obtain second detected image data. The first optical filter and the second optical filter are disposed on the first image detecting unit and the second image detecting unit, respectively. The passing wavelength of the first optical filter is substantially different from that of the second optical filter. The processing unit is configured to perform a differential operation on the first detected image data and the second detected image data, so as to obtain differential image data.一種生物攝像擷取裝置,包含發光元件、第一影像感測元件、第二影像感測元件、第一濾光元件、第二濾光元件和處理單元。發光元件用以朝向生物組織發射光源。第一影像感測元件用以對生物組織進行光感測,以得到第一感測影像資料。第二影像感測元件用以對生物組織進行光感測,以得到第二感測影像資料。第一濾光元件和第二濾光元件分別設置於第一影像感測元件和第二影像感測元件上。第二濾光元件與第一濾光元件之光穿透波長實質相異。處理單元用以對第一感測影像資料和第二感測影像資料進行差分運算處理,以得到差分影像資料。100‧‧‧生物攝像擷取裝置102‧‧‧殼體110A‧‧‧第一影像感測元件110B‧‧‧第二影像感測元件120A‧‧‧第一濾光元件120B‧‧‧第二濾光元件130‧‧‧發光元件140‧‧‧處理單元150‧‧‧通訊單元160‧‧‧電力接收單元162‧‧‧傳輸線170‧‧‧電源開關
