PROBLEM TO BE SOLVED: To provide a method for constructing a non-human model animal to which human cancer cells can be transplanted without suppressing the immunity of the model animal.SOLUTION: A method for constructing a model animal to which xenogeneic cells can be transplanted includes a step in which xenogeneic cells are transplanted to a small-sized model fish in a first period when the immune cells of the fish are in an undifferentiated state. The xenogeneic cells are human cancer cells, and are preferably either created by irradiating human cancer cells with γ rays or obtained by differentiating human cancer cells based on their surface antigens into a human cancer cell population in which the proportion of AlDH positive human cancer cells is 50 percent. In addition, the small-sized fish is a zebrafish, and the first period is preferably after a lapse of 30 to 72 hours from fertilization. The method for constructing a model animal to which xenogeneic cells can be transplanted also includes a step in which the xenogeneic cells having proliferative capacity are transplanted to the model animal again in a second period after a lapse of a predetermined period from the first period. The second period is after a lapse of 1 to 6 months from fertilization.COPYRIGHT: (C)2014,JPO&INPIT【課題】ヒトがん細胞を免疫抑制されることなく異種動物に移植可能なモデル動物の作製方法を提供する。【解決手段】モデル小型魚類の免疫細胞が未分化な状態にある第1の時期に、異種細胞を移植することを特徴とする異種細胞移植モデル動物の作製方法であって、前記異種細胞がヒトがん細胞で、γ線を照射して作られたもの又はヒトのガン細胞を表面抗原法により分画するか、又は、ヒトがん細胞集団中のAlDH(+)の割合が50%となったものかのいずれかであることが好ましく、また、前記小型魚類がゼブラフィッシュであって、前記第1の時期が受精後30時間~72時間経過後であることが好ましい。前記第1の時期から所定期間経過後の第2の時期に増殖機能を維持した前記異種細胞を再度移植する異種細胞移植モデル動物の作成方法。前記第2の時期は受精後1ヶ月~6ヶ月経過後である。【選択図】図3
