GÈNE SYNTHÉTIQUE POUR L'EXPRESSION DE SM-14 CHEZ PICHIA PASTORIS, PROCÉDÉS DE PRODUCTION ET DE PURIFICATION DE SM-14 ET SON UTILISATION COMME VACCIN ET À DES FINS DIAGNOSTIQUES
Fundação Oswaldo Cruz;Alvos - Consultoria, Desenvolvimento E Comercializ
发明人:
申请号：
EP11824375.7
公开号：
EP2617829A1
申请日:
2011.09.13
申请国别(地区):
EP
年份:
2013
代理人:
摘要：
The present invention is related to the field of recombinant protein production using a synthetic gene associated with high protein expression in Pichia pastoris. More specifically, this invention describes the production of Sm14 Schistosoma mansoni recombinant protein. A synthetic gene was created to promote high Sm14 expression, and with this gene we obtained and genetically manipulated a Pichia pastoris strain for effectively producing a vaccine. We have also improved that protein's production and purification processes from P. pastoris cells； the industrial production of such processes may be scheduled.
