The present invention relates to a lipid composition that self-emulsifies in the presence of a hydrophilic phase, the composition comprising from 0.001% to 20% by weight, based on the total weight of the composition, of at least one active substance； 5% to 60% by weight of an oily phase, based on the total weight of the composition； a first surfactant； a second surfactant, wherein the first surfactant and the second surfactant are combined. The ratio is 0.5 to 6, and the lipid composition forms particles having a charge of 10 mV to 100 mV as an absolute value after being dispersed in the hydrophilic phase. The present invention also relates to a method for preparing a self-emulsifying lipid composition, and to the use of said self-emulsifying lipid composition in the field of medicine or nutritional veterinary medicine. [Selection diagram] None本発明は、親水性相の存在下で自己乳化する脂質組成物に関し、該組成物は、組成物の総重量に対して、０．００１重量％～２０重量％の少なくとも１種の活性物質；組成物の総重量に対して、５重量％～６０重量％の油性相；第一界面活性剤；第２の界面活性剤を含み、第１の界面活性剤と第２の界面活性剤との比率は、０．５～６であり、前記脂質組成物は、親水性相に分散後、絶対値として、１０ｍＶ～１００ｍＶの電荷を有する粒子を形成する。本発明はまた、自己乳化型脂質組成物の調製方法、および医薬分野または栄養補助獣医学分野での前記自己乳化型脂質組成物の使用にも関する。【選択図】なし
