A novel promoter having an environmental stress-responding property and capable of acting specifically in a specific tissue can be used to induce the expression of a gene specifically in the specific tissue. Specifically disclosed is a tissue-specific gene expression method comprising the steps of: providing a plant having a gene located downstream to an environmental stress-responding promoter containing DNA selected from the following items (a), (b) and (c): (a) DNA which comprises a nucleotide sequence selected from those depicted in SEQ ID NOs:1 to 8 (b) DNA which comprises a nucleotide sequence having the deletion, substitution or addition of one or more nucleotides in a nucleotide sequence selected from those depicted in SEQ ID NOs:1 to 8 and can act as an environmental stress-responding promoter and (c) DNA which is capable of hybridizing with DNA comprising a nucleotide sequence selected from those depicted in SEQ ID NOs:1 to 8 under stringent conditions and can act as an environmental stress-responding promoter and cultivating the plant under environmental stress conditions. The method enables to induce the expression of the gene located downstream to the environmental stress-responding promoter in a tissue-specific manner.
