A puncture device includes a body, a barrel, a tube and two clamping parts; a clamping piece is installed on the body; the clamping piece has a clamping seam, two clamping parts are respectively clamped with the clamping part of the body at both ends of the long seam of the clamping seam; a puncture piece has a clamping seam of the clamping part which can be penetrated by the puncture part and inserted in the tube part of the body.Or, a puncture device, which comprises a body, a cylinder part, a tube part, a clamping sealing piece, which is arranged on the body, the clamping sealing piece has a clamping sealing seam, a first flap piece, which is arranged on the top of the clamping sealing piece, the first flap piece has a flap hole, a bunch of sealing pieces, which is arranged on the top of the first flap piece, the bundle sealing piece has a sealing hole, the puncture piece, which can be penetrated by the first flap piece A flap hole of a flap piece, a sealing hole of a bundle sealing piece, a clamping sealing seam of a clamping sealing piece, and a pipe inserted in the body.一種穿刺器,包含有一本體,具有一筒部,一管部,兩卡掣部;一夾封件,裝設於該本體;該夾封件具有一夾封縫,兩卡掣部位於夾封縫的長縫向兩端分別與該本體的卡掣部卡掣;一穿刺件,具有一穿刺部可穿設該夾封件的夾封縫及插設於該本體的管部。或者,一種穿刺器,包含有一本體,具有一筒部,一管部;一夾封件,裝設於該本體;該夾封件具有一夾封縫;一第一瓣件,設於該夾封件上方;該第一瓣件具有一瓣孔;一束封件,設於該第一瓣件上方;該束封件具有一封孔;一穿刺件,具有一穿刺部可穿設該第一瓣件的瓣孔、束封件的封孔、夾封件的夾封縫、及插設於該本體的管部。