A biological observation apparatus according to an aspect of the present invention includes a sound wave radiating unit that radiates a sound wave into an object to be examined, a light radiating unit that radiates first light having a predetermined wavelength and second light different from the first light into a portion of the object influenced by the sound wave, a detector that detects reflected light of the first light and reflected light of the second light, and a calculation unit that calculates characteristic information of the object based on the reflected light of the first light and the reflected light of the second light.