Provided are pharmaceutical formulations comprising a recombinant acid α-glucosidase, wherein the recombinant acid α-glucosidase is expressed in Chinese hamster ovary (CHO) cells and comprises an increased content of N-glycan units bearing one or two mannose-6-phosphate residues when compared to a content of N-glycan units bearing one or two mannose-6-phosphate residues of alglucosidase alfa at least one buffer selected from the group consisting of a citrate, a phosphate and combinations thereof and at least one excipient selected from the group consisting of mannitol, polysorbate 80, and combinations thereof, wherein the formulation has a pH of from about 5.0 to about 7.0. Also provided are methods of treating Pompe disease using these pharmaceutical formulations.提供了包含重組酸性α-葡萄糖苷酶的藥物調配物,其中該重組酸性α-葡萄糖苷酶在中國倉鼠卵巢(CHO)細胞中表現,並且當相比於帶有阿葡萄糖苷酶α的一個或兩個甘露糖-6-磷酸殘基的N-聚糖單元的含量時,該重組酸性α-葡萄糖苷酶包括增加的含量的帶有一個或兩個甘露糖-6-磷酸殘基的N-聚糖單元;至少一種選自下組的緩衝液,該組由以下各項組成:檸檬酸鹽、磷酸鹽及其組合;和至少一種選自下組的賦形劑,該組由以下各項組成:甘露醇、聚山梨醇酯80及其組合,其中該調配物具有從約5.0至約7.0的pH。亦提供了使用該等藥物調配物治療龐貝氏病之方法。
