The invention provides a new method for evaluating transfer embryos including blastocysts used for in vitro fertilization in fertility treatment, and a method for evaluating transfer embryos using a new biomarker necessary for evaluation. The method comprises the steps of (a) providing a test object, for example, a culture solution of a human blastocyst, containing norepinephrine (noradrenaline) released from a transfer embryo, such as a human blastocyst, obtained from a subject (b) quantitatively analyzing norepinephrine in the test object by a combination of ultra high performance liquid chromatography and mass spectrometry or the like (c) predicting the quality of the transfer embryo based on the amount of norepinephrine from analysis results obtained and (d) transferring the embryo into a suitable female recipient for implantation, if the transfer embryo is predicted to be of good quality and/or to lead to the establishment of a viable pregnancy based on step (c).
