This invention relates to a biopsy instrument which is self-cutting, self-retrieval of the tissue. This invention relates to a biopsy instrument which includes a punch cutting element with an inner surface, an outer surface and a cutting edge along the bottom surface. This invention also defines a hollow space between the inner surfaces where the desired tissue is punched. Furthermore, this invention also relates to a new design of the instrument where the punch cutting blades ensure more accurate and precised tissue cut ranging from 3mm to 8mm of depth. The biopsy instrument also includes a horizontal cutting element which is placed along the inner surface of the punch cutting element and is configured to detach the final biopsy tissue from the underlying tissue after the desired depth of the tissue is punched with minimum tissue architectural damage. This design of the instrument also includes small led lights for better illumination of the biopsy site. After retrieval, the tissue is transferred directly to formal saline for preservation by the downward movement of a piston which gives a downward force to the tissue. This invention also provides an instrument which is precise, efficient, user friendly.