Zhiyuan GONG,Jiangyan HE,Bensheng JU,Toong Jin LAM,Yanfei XU,Tie YAN
申请号：
US15674798
公开号：
US20180064074A1
申请日:
2017.08.11
申请国别(地区):
US
年份:
2018
代理人:
摘要：
Four zebrafish gene promoters, which are skin specific, muscle specific, skeletal muscle specific and ubiquitously expressed respectively, were isolated and ligated to the 5′ end of the EGFP gene. When the resulting chimeric gene constructs were introduced into zebrafish, the transgenic zebrafish emit green fluorescence under a blue light or ultraviolet light according to the specificity of the promoters used. Thus, new varieties of ornamental fish of different fluorescence patterns, e.g., skin fluorescence, muscle fluorescence, skeletal muscle-specific and/or ubiquitous fluorescence, are developed.
