An image pickup unit for endoscope is configured such that a first lens frame and a second lens frame are fitted to each other with a first fitting area and bonded and fixed to each other with a first adhesive at a first frame fixing portion, the second lens frame and a holding frame are fitted to each other with a second fitting area and bonded and fixed to each other with a second adhesive at a second frame fixing portion, the first lens frame, the second lens frame, and the fixing frame are bonded and fixed together with the second adhesive, the second fitting area is larger than the first fitting area, and the second adhesive has a glass transition temperature lower than a glass transition temperature of the first adhesive.